Aptamer and bifunctional enzyme co-functionalized MOF-derived porous carbon for low-background electrochemical aptasensing.

Hui Wan, Xiyue Cao, Min Liu, Feifei Zhang, Chao Sun, Jianfei Xia, Zonghua Wang

Analytical and bioanalytical chemistry 2021 Oct

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To improve the efficiency of aptasensors, a signal amplification strategy by coupling tyrosinase (Tyr)-triggered redox cycling with nanoscale porous carbon (NCZIF) has been proposed. The NCZIF was obtained by calcining ZIF-8 crystals in an inert atmosphere. It had high surface areas, great biocompatibility, and ease of functionalization, which was beneficial for immobilizing sufficient Tyr and aptamer covalently. When the target prostate-specific antigen (PSA) was present, the NCZIF functionalized with Tyr and an aptamer bound to the aptamer-modified Au electrode specifically through the sandwich structure. Then, Tyr acted to oxidize the electroinactive phenol, which led to low-background signal, in the substrate to electroactive catechol, and triggered the redox cycling under the action of NADH. The low detection limit of the proposed electrochemical aptasensor for PSA was 0.01 ng mL-1, and the wide detection range was from 0.01 to 50 ng mL-1. The use of ZIF-8 derived porous carbon and Tyr-triggered redox cycling system provided a promising solution for the development of simple, rapid, reliable, and low-background aptasensing methods, which had great potential in the field of disease diagnosis and biomedicine. © 2021. Springer-Verlag GmbH Germany, part of Springer Nature.

Citation

Hui Wan, Xiyue Cao, Min Liu, Feifei Zhang, Chao Sun, Jianfei Xia, Zonghua Wang. Aptamer and bifunctional enzyme co-functionalized MOF-derived porous carbon for low-background electrochemical aptasensing. Analytical and bioanalytical chemistry. 2021 Oct;413(25):6303-6312