Generation of a homozygous ZBTB7A knockout human induced pluripotent stem line by CRISPR/Cas9 editing.

Diyu Chen, Bing Song, Yi Cheng, Lifen Zhu, Dian Lu, Nengqing Liu, Yinghong Yang, Xiaofang Sun

Stem cell research 2021 Aug

filter terms:

ZBTB7A plays important roles in several biological processes, including silencing of the fetal γ-globin genes, hematopoiesis, primed-to-naive transition, etc. Meanwhile, it is also associated with Oncogenic transformation and tumor progression. However, the mechanism of ZBTB7A function is not fully understood yet. Here, we generated a homozygous ZBTB7A knockout human induced pluripotent stem cell (iPSC) line, GZHMCi007-A by the CRISPR/Cas9-mediated homology-dependent DNA repair method. The iPSCs of ZBTB7A-/- established by us is a powerful tool for related research. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Citation

Diyu Chen, Bing Song, Yi Cheng, Lifen Zhu, Dian Lu, Nengqing Liu, Yinghong Yang, Xiaofang Sun. Generation of a homozygous ZBTB7A knockout human induced pluripotent stem line by CRISPR/Cas9 editing. Stem cell research. 2021 Aug;55:102494