Detection of Long Non-coding RNA Expression by Non-radioactive Northern Blots.

Yueying Wang, Mu Xu, Jiao Yuan, Zhongyi Hu, Youyou Zhang, Lin Zhang, Xiaowen Hu

Methods in molecular biology (Clifton, N.J.) 2021

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With the advances in sequencing technology and transcriptome analysis, it is estimated that up to 75% of the human genome is transcribed into RNAs. This finding prompted intensive investigations on the biological functions of non-coding RNAs and led to very exciting discoveries of microRNAs as important players in disease pathogenesis and therapeutic applications. Research on long non-coding RNAs (lncRNAs) is in its infancy, yet a broad spectrum of biological regulations has been attributed to lncRNAs. As a novel class of RNA transcripts, the expression level and splicing variants of lncRNAs are various. Northern blot analysis can help us learn about the identity, size, and abundance of lncRNAs. Here we describe how to use northern blot to determine lncRNA abundance and identify different splicing variants of a given lncRNA. © 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Yueying Wang, Mu Xu, Jiao Yuan, Zhongyi Hu, Youyou Zhang, Lin Zhang, Xiaowen Hu. Detection of Long Non-coding RNA Expression by Non-radioactive Northern Blots. Methods in molecular biology (Clifton, N.J.). 2021;2372:145-156