microRNA-4429-5p suppresses the malignant development of colon cancer by targeting matrix metalloproteinase 16.

Wei Li, Zhe Song, Nan Jia, Cui Zhang, Weina Gao, Liang Wang

In vitro cellular & developmental biology. Animal 2021 Aug

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Colon cancer has been recognized as the major reason for global cancer-associated mortality. microRNA (miRNA, miR)-4429-5p has been documented to act as a tumor-suppressive miRNA in some cancers, but its effect on colon cancer remains elusive. In this study, the biological effects of miR-4429-5p were investigated both in vitro by MTT, 5-ethynyl-2'-deoxyuridine (EdU), wound healing, and transwell assays and in vivo by a xenograft mice model. Western blot, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and dual-luciferase assay were used to identify the binding of miR-4429-5p on matrix metalloproteinase 16 (MMP16) 3'-UTR. Our results suggested that overexpression of miR-4429-5p hindered colon cancer cell proliferation, migration, and invasion, whereas knockdown of miR-4429-5p exhibited the opposite effect in colon cancer cells. Mechanistically, miR-4429-5p directly bound to the 3'-UTR of MMP16 and led to inhibition of MMP16 protein. Overexpression of miR-4429-5p inhibited colon tumor growth by targeting MMP16. Taken together, our study revealed that miR-4429-5p prevented colon cancer progression through targeting MMP16, indicating miR-4429-5p as a promising target for treatment improvement for colon cancer. © 2021. The Society for In Vitro Biology.

Citation

Wei Li, Zhe Song, Nan Jia, Cui Zhang, Weina Gao, Liang Wang. microRNA-4429-5p suppresses the malignant development of colon cancer by targeting matrix metalloproteinase 16. In vitro cellular & developmental biology. Animal. 2021 Aug;57(7):715-725