Cell wall proteomic analysis of the cnidarian photosymbionts Breviolum minutum and Cladocopium goreaui.

Giada Tortorelli, Clinton A Oakley, Simon K Davy, Madeleine J H van Oppen, Geoffrey I McFadden

The Journal of eukaryotic microbiology 2022 Jan

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The algal cell wall is an important cellular component that functions in defense, nutrient utilization, signaling, adhesion, and cell-cell recognition-processes important in the cnidarian-dinoflagellate symbiosis. The cell wall of symbiodiniacean dinoflagellates is not well characterized. Here, we present a method to isolate cell walls of Symbiodiniaceae and prepare cell-wall-enriched samples for proteomic analysis. Label-free liquid chromatography-electrospray ionization tandem mass spectrometry was used to explore the surface proteome of two Symbiodiniaceae species from the Great Barrier Reef: Breviolum minutum and Cladocopium goreaui. Transporters, hydrolases, translocases, and proteins involved in cell-adhesion and protein-protein interactions were identified, but the majority of cell wall proteins had no homologues in public databases. We propose roles for some of these proteins in the cnidarian-dinoflagellate symbiosis. This work provides the first proteomics investigation of cell wall proteins in the Symbiodiniaceae and represents a basis for future explorations of the roles of cell wall proteins in Symbiodiniaceae and other dinoflagellates. © 2021 The Authors. Journal of Eukaryotic Microbiology published by Wiley Periodicals LLC on behalf of International Society of Protistologists.

Citation

Giada Tortorelli, Clinton A Oakley, Simon K Davy, Madeleine J H van Oppen, Geoffrey I McFadden. Cell wall proteomic analysis of the cnidarian photosymbionts Breviolum minutum and Cladocopium goreaui. The Journal of eukaryotic microbiology. 2022 Jan;69(1):e12870