Multi-Locus Sequence Typing and Lipooligosaccharide Class Analysis of Campylobacter jejuni HS:19 Isolated in Japan.

Satoru Akase, Keiko Yokoyama, Hiromi Obata, Chie Monma, Noriko Konishi, Kaoru Hatakeyama, Dai Saiki, Masako Maeda, Chikako Asayama, Jun Suzuki, Kenji Sadamasu

Japanese journal of infectious diseases 2022 Mar 24

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Campylobacter jejuni is a major foodborne pathogen that causes enteritis in humans, and is also known to be an antecedent infectious factor for Guillain-Barré syndrome (GBS). The onset of GBS after C. jejuni infection results from molecular mimicry between human neuronal gangliosides and C. jejuni lipooligosaccharides (LOS). C. jejuni HS:19 has been previously isolated from GBS cases more frequently than other serotypes in Japan. Therefore, in this study, we performed molecular analysis of 88 HS:19 isolates from GBS cases, sporadic diarrhea patients, and poultry meat samples, using multi-locus sequence typing and LOS class analysis. As a result, 87 of the 88 HS:19 isolates were typed as ST22 / CC22 and LOS class A1, while one was typed as ST1947 / CC22 and LOS class A1. Furthermore, the analysis of another 331 isolates from sporadic enteritis cases showed that only 34 (10.3%) were classified as LOS class A, including HS:19 (25 isolates), HS:2 (8 isolates), and HS:4c (1 isolate). In conclusion, C. jejuni HS:19 had high clonality, regardless of its origin, compared to other capsule types in Japan.

Citation

Satoru Akase, Keiko Yokoyama, Hiromi Obata, Chie Monma, Noriko Konishi, Kaoru Hatakeyama, Dai Saiki, Masako Maeda, Chikako Asayama, Jun Suzuki, Kenji Sadamasu. Multi-Locus Sequence Typing and Lipooligosaccharide Class Analysis of Campylobacter jejuni HS:19 Isolated in Japan. Japanese journal of infectious diseases. 2022 Mar 24;75(2):199-201