Naked cuticle inhibits wingless signaling in Drosophila wing development.

Rui Wang, Hao Xie, Lin Yang, Ping Wang, Meng-Meng Chen, Hong-Yan Wu, Yan-Lin Liao, Ming-Ying Wang, Qiang Wang, Xiao-Xia Gong, Qian Cheng, Lin Cheng, Fei-Yan Xie, Cai-Li Bi, Ming Fang

Biochemical and biophysical research communications 2021 Oct 22

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Wnt signaling is one of the major signaling pathways that regulate cell differentiation, tissue patterning and stem cell homeostasis and its dysfunction causes many human diseases, such as cancer. It is of tremendous interests to understand how Wnt signaling is regulated in a precise manner both temporally and spatially. Naked cuticle (Nkd) acts as a negative-feedback inhibitor for Wingless (Wg, a fly Wnt) signaling in Drosophila embryonic development. However, the role of Nkd remains controversial in later fly development, particularly on the canonical Wg pathway. In the present study, we show that nkd is essential for wing pattern formation, such that both gain and loss of nkd result in the disruption of Wg target expression in larvae stage and abnormal adult wing morphologies. Furthermore, we demonstrate that a thirty amino acid fragment in Nkd, identified previously in Wharton lab, is critical for the canonical Wg signaling, but is dispensable for Wg/planar cell polarity pathway. Putting aside the pleiotropic nature of nkd function, i.e. its role in the Decapentaplegic signaling, we conclude that Nkd universally inhibits the canonical Wg pathway across a life span of Drosophila development. Copyright © 2021 Elsevier Inc. All rights reserved.

Citation

Rui Wang, Hao Xie, Lin Yang, Ping Wang, Meng-Meng Chen, Hong-Yan Wu, Yan-Lin Liao, Ming-Ying Wang, Qiang Wang, Xiao-Xia Gong, Qian Cheng, Lin Cheng, Fei-Yan Xie, Cai-Li Bi, Ming Fang. Naked cuticle inhibits wingless signaling in Drosophila wing development. Biochemical and biophysical research communications. 2021 Oct 22;576:1-6