Keyan Peterson, Stephanie Coffman, Stacey Wolfe, Zhidan Xiang
Journal of neuroscience methods 2021 Nov 01Latex perfusion is an effective tool to study cerebrovascular pathology in the animal brain. It provides, low-cost, high fidelity anatomical information on ex-vivo analysis, and can be utilized to study multiple, states. However, current methods of latex casting and tissue-clearance do not allow for immunohistochemical analysis following sample processing. This results in experiments that require increased numbers of animals to attain adequate data. In this paper, we present a modified latex perfusion and tissue processing protocol for ex-vivo analysis, of the cerebral vasculature. The method consists of injection of the arterial tree with liquid latex, followed by tissue clearance with a scale solution. Our results demonstrate effective and reliable perfusion of the murine cerebrovascular tree, rendering the arterial morphology of the brain in high detail, while allowing for post-perfusion, immunohistochemistry of the sample. Our technique bypasses the limitations of previous latex angiography protocols by allowing for postperfusion, pathologic analysis of casted cerebrovascular tissue. This protocol provides a reliable, low-cost, method of cerebrovascular perfusion that reduces the number of animals required to generate robust data from latex-casted brain tissue. Copyright © 2021 Elsevier B.V. All rights reserved.
Keyan Peterson, Stephanie Coffman, Stacey Wolfe, Zhidan Xiang. A novel method for ex-vivo latex angiography of the mouse brain. Journal of neuroscience methods. 2021 Nov 01;363:109342
PMID: 34478766
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