Genome sequence and geographic distribution of a new nepovirus infecting Stenotaphrum secundatum in Australia.

Nga T Tran, Ai Chin Teo, Kathleen S Crew, Paul R Campbell, John E Thomas, Andrew D W Geering

Virus research 2021 Nov

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The genome sequence of a new subgroup C nepovirus from Stenotaphrum secundatum in Australia is described. This virus, tentatively named Stenotaphrum nepovirus (SteNV), was present in separate plants as a mixed infection with either sugarcane mosaic virus or Panicum mosaic virus. The virus genome was divided between two RNA segments, 7,824 and 7,104 nucleotides (nt) in length, which each encode a single long polyprotein with putative 3C-like cysteine protease sites of the type H/G, H/S or L/S. The 3' untranslated region of RNA2, at 2,155 nt, is the longest observed for any subgroup C nepovirus. Phylogenetic analyses using protease-polymerase and coat protein amino acid alignments suggest that SteNV is most closely related to cherry leaf roll virus. Using a newly developed RT-PCR assay, this virus was detected at multiple localities in New South Wales, Queensland and Western Australia, and in a second host species, Digitaria didactyla. No consistent association between virus infection and symptoms could be established. The economic importance, pathogenicity and transmission of this novel virus species warrant further investigation. Copyright © 2021. Published by Elsevier B.V.

Citation

Nga T Tran, Ai Chin Teo, Kathleen S Crew, Paul R Campbell, John E Thomas, Andrew D W Geering. Genome sequence and geographic distribution of a new nepovirus infecting Stenotaphrum secundatum in Australia. Virus research. 2021 Nov;305:198554