Poonam Kumari, Sujoy Bowmik, Sudipto Kumar Paul, Bidisha Biswas, Sanjay K Banerjee, Upadhyayula Surayanarayana Murty, Velayutham Ravichandiran, Utpal Mohan
Biotechnology and bioengineering 2021 DecSortase A, a transpeptidase enzyme is present in many Gram-positive bacteria and helps in the recruitment of the cell surface proteins. Over the last two decades, Sortase A has become an attractive tool for performing in vivo and in vitro ligations. Sortase A-mediated ligation has continuously been used for its specificity, robustness, and highly efficient nature. These properties make it a popular choice among protein engineers as well as researchers from different fields. In this review, we give an overview of Sortase A-mediated ligation of various molecules on the cell surfaces, which can have diverse applications in interdisciplinary fields. © 2021 Wiley Periodicals LLC.
Poonam Kumari, Sujoy Bowmik, Sudipto Kumar Paul, Bidisha Biswas, Sanjay K Banerjee, Upadhyayula Surayanarayana Murty, Velayutham Ravichandiran, Utpal Mohan. Sortase A: A chemoenzymatic approach for the labeling of cell surfaces. Biotechnology and bioengineering. 2021 Dec;118(12):4577-4589
PMID: 34491580
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