In Silico Screening of Attractive Target Genes for RNAi-Based Pest Control.

Currently, RNAi-based techniques are emerging as powerful tools for pest control. In RNAi strategies for pest control, screening efficient RNAi target genes is a crucial initial step. In this chapter, I describe the crucial procedure of in silico screening of the most a5ttractive target genes for RNAi-based pest control, including gene or protein homology comparison, specificity optimization of the dsRNAs to target pests to minimize off-target effect, and primer design. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Weilin Zhang. In Silico Screening of Attractive Target Genes for RNAi-Based Pest Control. Methods in molecular biology (Clifton, N.J.). 2022;2360:75-83

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PMID: 34495508

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