Examination of the Suitability of Attractive Target Genes for RNAi-Based Pest Control.

Weilin Zhang

Methods in molecular biology (Clifton, N.J.) 2022

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In the past two decades, studies investigating RNAi-based pest control have continued as a major focus of research. In this chapter, I describe the crucial procedure of examining the suitability of attractive target genes in insects for RNAi-based pest control, including preliminarily examining the suitability of the candidate genes by gene expression analysis, encapsulating dsRNA using nanoparticles to avoid degradation caused by insect digestive systems, and introducing exogenous dsRNAs into pests through micro-injection to rapidly and effectively examine the suitability of dsRNA in vivo in insects. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Weilin Zhang. Examination of the Suitability of Attractive Target Genes for RNAi-Based Pest Control. Methods in molecular biology (Clifton, N.J.). 2022;2360:175-185