Expansion of Human iNKT Cells Ex Vivo.

Invariant natural killer T (iNKT) cells are credited with antitumor activity by preclinical studies and clinical trials. Efficient expansion of iNKT cells ex vivo is essential for their translational usage. The culturing procedure described here provides an optimized method for ex vivo expansion of iNKT cells using recombinant human IL-15 (rhIL-15) and recombinant human IL-12 (rhIL-12), which results in cell products with enhanced cytokine secretion and cytotoxicity while maintaining the purity and viability of iNKT cells.© 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Jing Wang, Chen Zhao, Jianqing Xu. Expansion of Human iNKT Cells Ex Vivo. Methods in molecular biology (Clifton, N.J.). 2021;2388:123-129

PMID: 34524667

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