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Soluble forms of cytokine receptors can be involved in the endogenous regulation of cytokine activity. Soluble interleukin 7 receptor α (sCD127) naturally binds IL-7, therefore there is interest in its potential application as an immunotherapeutic agent to regulate IL-7. With the hypothesis that sCD127 enhances IL-7 activity, thus promoting T-cell proliferation in vivo, we sought to assess the effect of sCD127, IL-7 or IL-7 + sCD127 treatment on CD4+ and CD8+ T-cells in the blood and spleen of mice. Peripheral blood mononuclear cells and splenocytes were prepared, and analyzed for T-cell number, phenotype and proliferation (Ki67+ ) by flow cytometry. IL-7 treatment induced T-cell proliferation, increased T-cell number, and triggered T-cell differentiation each of which was enhanced with the addition of sCD127. IL-7 + sCD127 treatment significantly increased spleen weight over that seen with IL-7 treatment alone. More pronounced proliferation and a greater increase in cell number was observed in CD8+ T-cells relative to the effect on CD4+ T-cells. These findings suggest that the addition of sCD127 enhances IL-7-mediated T-cell proliferation and suggests a potential therapeutic use for sCD127. © 2021 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.

Citation

Nawaf A Aloufi, Alaa K Ali, Stephanie C Burke Schinkel, Bengisu Molyer, Priscila O Barros, Joanne E McBane, Seung-Hwan Lee, Jonathan B Angel. Soluble CD127 potentiates IL-7 activity in vivo in healthy mice. Immunity, inflammation and disease. 2021 Dec;9(4):1798-1808

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PMID: 34525268

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