Snake Venom Identification via Fluorescent Discrimination.

Fei Chen, Meng Qin, Wei Liu, Fan Wang, Wanjie Ren, Huihua Xu, Fengyu Li

Analytical chemistry 2021 Oct 26

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The identification and discrimination of snake venom are highly desired for timely clinical treatment. However, the complex components in snake venom make it a great challenge to achieve rapid and accurate identification. Inspired by the organism's taste sensing system, a fluorescent sensor array that could differentiate snake venoms was fabricated. The interaction of snake venoms with different fluorescent dyes in the sensor array gave rich information, based on which efficient detection of complex snake venom was achieved. The main six proteins of snake venom in the same concentration, different concentrations, and their mixtures were identified with 100% accuracy. Furthermore, seven snake venoms belonging to different snake families were discriminated in PBS buffer and human plasma. Interferents of bovine serum albumin (BSA), thrombin, and transferrin (TRF) demonstrated the practicability of the fluorescent sensor array. This strategy of a multiresponse sensor array provides an effective method for accurate and rapid venom toxicology analysis, benefiting early and timely clinical diagnosis and treatment.

Citation

Fei Chen, Meng Qin, Wei Liu, Fan Wang, Wanjie Ren, Huihua Xu, Fengyu Li. Snake Venom Identification via Fluorescent Discrimination. Analytical chemistry. 2021 Oct 26;93(42):14025-14030