Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells.

Cierra N Sing, Emily J Yang, Ryo Higuchi-Sanabria, Liza A Pon, Istvan R Boldogh, Theresa C Swayne

Methods in molecular biology (Clifton, N.J.) 2022

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Budding yeast, Saccharomyces cerevisiae, is an appealing model organism to study the organization and function of the actin cytoskeleton. With the advent of techniques to perform high-resolution, multidimensional analysis of the yeast cell, imaging of yeast has emerged as an important tool for research on the cytoskeleton. This chapter describes techniques and approaches for visualizing the actin cytoskeleton in fixed yeast cells with wide-field and super-resolution fluorescence microscopy. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Cierra N Sing, Emily J Yang, Ryo Higuchi-Sanabria, Liza A Pon, Istvan R Boldogh, Theresa C Swayne. Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells. Methods in molecular biology (Clifton, N.J.). 2022;2364:81-100