Studying Hedgehog Signaling During Mouse Neural Tube Development.

Sandii Constable, Kevin White, Bandarigoda Nipunika Somatilaka, Saikat Mukhopadhyay

Methods in molecular biology (Clifton, N.J.) 2022

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The identity of ventral neural progenitors in the neural tube is largely dependent on Hedgehog (Hh) signaling. Variations in staining patterns are excellent indicators of aberrant Hh signaling. Here we describe the basic protocol to stain for progenitor populations based on transcription factor expression. We also provide an overview of ciliary and centrosomal staining in the neural tube. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Sandii Constable, Kevin White, Bandarigoda Nipunika Somatilaka, Saikat Mukhopadhyay. Studying Hedgehog Signaling During Mouse Neural Tube Development. Methods in molecular biology (Clifton, N.J.). 2022;2374:59-71