Protocol for chemically induced murine gastric tumor model.

Ke Li, Ao Wang, Huijuan Liu, Baojie Li

STAR protocols 2021 Dec 17

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N-Methyl-N-nitrosourea, an N-nitroso compound converted from dietary nitrite by Helicobacter pylori, causes somatic mutations in epithelial cells and induces gastric premalignancy. Here, we describe a detailed protocol for induction of gastric tumor and analysis of tumor phenotypes in mice. This model can be widely used for studying the initiation and growth of gastric cancer. For complete details on the use and execution of this protocol, please refer to Li et al. (2021). © 2021 The Authors.