In Situ Fabrication and Perfusion of Tissue-Engineered Blood Vessel Microphysiological System.

Xu Zhang, Nadia O Abutaleb, Ellen Salmon, George A Truskey

Methods in molecular biology (Clifton, N.J.) 2022

filter terms:

Human tissue-engineered blood vessels (TEBVs) that exhibit vasoactivity can be used to test drug toxicity, modulate pro-inflammatory cytokines, and model disease states in vitro. We developed a novel device to fabricate arteriole-scale human endothelialized TEBVs in situ with smaller volumes and higher throughput than previously reported. Both primary and induced pluripotent stem cell (iPSC)-derived cells can be used. Four collagen TEBVs with 600μm inner diameter and 2.9 mm outer diameter are fabricated by pipetting a solution of collagen and medial cells into a three-layer acrylic mold. After gelation, the TEBVs are released from the mold and dehydrated. After suturing the TEBVs in place and changing the mold parts to form a perfusion chamber, the TEBVs are endothelialized in situ, and then media is perfused through the lumen. By removing 90% of the water after gelation, the TEBVs become mechanically strong enough for perfusion at the physiological shear stress of 0.4 Pa within 24 h of fabrication and maintain function for at least 5 weeks. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Xu Zhang, Nadia O Abutaleb, Ellen Salmon, George A Truskey. In Situ Fabrication and Perfusion of Tissue-Engineered Blood Vessel Microphysiological System. Methods in molecular biology (Clifton, N.J.). 2022;2375:77-90