Detection of four foodborne pathogens based on magnetic separation multiplex PCR and capillary electrophoresis.

Shiyu He, Yanzhi Huang, Yingwei Ma, Haoyan Yu, Bo Pang, Xingxing Liu, Caihong Yin, Xiaomu Wang, Yuan Wei, Yuling Tian, Chao Zhao, Kun Xu, Juan Wang, Chunping Lv, Xiuling Song, Minghua Jin

Biotechnology journal 2022 Jan

filter terms:

analysis food (1)

electrophoresis (4)

food (2)

pcr (3)

Sizes of these terms reflect their relevance to your search.

Foodborne pathogen contamination is a major safety issue for many foods and is causing concern worldwide. In this study, a detection system based on magnetic separation, multiplex PCR (MPCR) and capillary electrophoresis (CE) technologies was developed for the simultaneous detection of four foodborne pathogens. Magnetic separation technology is used to rapidly capture pathogenic bacteria in food samples, and then a combination of MPCR and CE can be used to greatly increase detection sensitivity. The detection limit for bacterial DNA reached 10-5 -10-7 ng μL-1 and in the analysis of mocked food samples, the assay showed good sensitivity for bacterial detection ranging from 101 to 105 CFU mL-1 with excellent specificity. Compared to similar detection methodologies, this technique avoids the need for time-consuming enrichment cultures, is more sensitive, and can be used to assay simultaneously four foodborne pathogens. © 2021 Wiley-VCH GmbH.

Citation

Shiyu He, Yanzhi Huang, Yingwei Ma, Haoyan Yu, Bo Pang, Xingxing Liu, Caihong Yin, Xiaomu Wang, Yuan Wei, Yuling Tian, Chao Zhao, Kun Xu, Juan Wang, Chunping Lv, Xiuling Song, Minghua Jin. Detection of four foodborne pathogens based on magnetic separation multiplex PCR and capillary electrophoresis. Biotechnology journal. 2022 Jan;17(1):e2100335