FITC characterization of a cathepsin B-responsive nanoprobe for report of differentiation of HL60 cells into macrophages.

Chengxing Zhang, Jingjing Meng, Yanhui Zhang, Dehua Huang, Peiyang Yan, Bo Tan, Haizhen Jiang, Zongwu Deng

Journal of peptide science : an official publication of the European Peptide Society 2022 Mar

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A cathepsin B (Cat B)-responsive optical nanoprobe is designed and prepared for report of HL60 differentiation into macrophage. A peptide sequence FRFK is linked to fluorescein (FITC) via the distant amino group of its lysine and N-terminated with acrylic acid (AA) to yield a molecular fluorescent probe AA-FRFK (FITC). The molecular probe is further embedded in poly(lactic-co-glycolic acid) (PLGA) to form a fluorescent nanoprobe AA-FRFK (FITC)@PLGA. The resultant optical nanoprobe is degradable by lysosomal Cat B, which is expressed in macrophages with a level of 5-10 times of that in HL60 cells. As a result, a significant decrease in fluorescence intensity is associated with the differentiation process of HL60 to macrophage and can be used as an indication of the differentiation process. The findings may pave a way toward the development of a universal in vitro labeling strategy of exogenous stem cells for report of in vivo cell differentiation by a dual-mode imaging modality involving optical imaging and magnetic resonance imaging. © 2021 European Peptide Society and John Wiley & Sons, Ltd.

Citation

Chengxing Zhang, Jingjing Meng, Yanhui Zhang, Dehua Huang, Peiyang Yan, Bo Tan, Haizhen Jiang, Zongwu Deng. FITC characterization of a cathepsin B-responsive nanoprobe for report of differentiation of HL60 cells into macrophages. Journal of peptide science : an official publication of the European Peptide Society. 2022 Mar;28(3):e3371