MiR-122 radiosensitize hepatocellular carcinoma cells by suppressing cyclin G1.

Gang Xu, Shanshan Bu, Xiushen Wang, Hong Ge

International journal of radiation biology 2022

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Emerging evidence has shown that radiotherapy is an effective treatment for hepatocellular carcinoma (HCC), Micro(mi)RNAs are involved in regulating radiosensitivity in many cancers. MiR-122 accounts for approximately 70% of all cloned miRNAs in the liver, but there are few reports about whether it is involved in regulating of radiosensitivity in HCC cells. HCC cells (HepG2 and Huh7) overexpressing miR-122 were constructed by transfecting them with lentiviral-miR-122. Then, their proliferation ability was analyzed by the MTT, and colony formation assays and a xenograft tumor model was used to detect their radiosensitivity. The expression of cyclin G1 mRNA and protein was detected by the quantitative real-time polymerase chain reaction and western blotting, respectively. Overexpression of miR-122 inhibited the proliferation of, and radiosensitized HCC cells. Cyclin G1 mRNA and protein level were suppressed in HepG2 tumors overexpression miR-122. MiR-122 may be useful as a potential radiosensitizer for HCC, and its mechanism is related to the regulation of cyclin G1.

Citation

Gang Xu, Shanshan Bu, Xiushen Wang, Hong Ge. MiR-122 radiosensitize hepatocellular carcinoma cells by suppressing cyclin G1. International journal of radiation biology. 2022;98(1):11-17