Chemical Approaches to Prepare Modified Heparin and Heparosan Polymers for Biological Studies.

Karthik Raman, Sailaja Arungundram

Methods in molecular biology (Clifton, N.J.) 2022

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Heparin is a potent clinically used anticoagulant. It is a heterogeneous mixture of polymers that contain a variety of sulfation patterns. Heparin polymers carrying rare 3-O-sulfated glucosamine units have been proven to be critical for binding to antithrombin and elicit an anticoagulant response. Heparins with other sulfation patterns are able to bind to a variety of other proteins such as FGF, VEGF, and CXCL-3. By modulating heparin's sulfation pattern, it is possible to generate polymers that can regulate biological processes beyond hemostasis. In this chapter, we describe a variety of chemical modification methods, including N-acetylation, N-deacetylation, N-sulfation, O-sulfation, selective 2-O desulfation, and complete desulfation, to prepare heparin-like polymers with distinct sulfation patterns for conducting biological studies. © 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Karthik Raman, Sailaja Arungundram. Chemical Approaches to Prepare Modified Heparin and Heparosan Polymers for Biological Studies. Methods in molecular biology (Clifton, N.J.). 2022;2303:289-296