Enzymatic characterization of agmatinase (AGM-1) from the filamentous fungus Neurospora crassa.

Luis L Pérez-Mozqueda, Rafael Vazquez-Duhalt, Ernestina Castro-Longoria

Fungal genetics and biology : FG & B 2021 Dec

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Agmatinase is a metallohydrolase involved in the hydrolysis of agmatine to produce urea and putrescine. Although its role in organisms is still under study, there are no reports of this family of enzymes in filamentous fungi. Recently, a protein showing agmatinase activity was reported in Neurospora crassa. Therefore, the aim of this work is to determine if the protein (AGM-1) found in the filamentous fungus N. crassa is a true agmatinase. The protein AGM-1was purified directly from N. crassa cultures, and its enzymatic characterization was carried out. The catalytic parameters such as optimum pH, thermostability, transformation kinetics, and activity in the presence of a cofactor were determined. The results show that AGM-1 can use manganese as a cofactor for its enzymatic activity, showing a transformation rate constant (kcat) of 77 s-1 and an affinity constant (KM) of 50.5 mM. The protein loses 50% of its activity when incubated 15 min at 30 °C and reaches maximal enzymatic activity at a pH range of 8-8.5. Our results indicate that the AGM-1 from N. crassa shows similar characteristics to true agmatinases already reported in other organisms. Thus, our findings strongly support that the protein annotated as hypothetical agmatinase in N. crassa is a true agmatinase. Copyright © 2021 Elsevier Inc. All rights reserved.

Citation

Luis L Pérez-Mozqueda, Rafael Vazquez-Duhalt, Ernestina Castro-Longoria. Enzymatic characterization of agmatinase (AGM-1) from the filamentous fungus Neurospora crassa. Fungal genetics and biology : FG & B. 2021 Dec;157:103634