Suppressors of cytokine signaling 1 protein in a regenerative model of the Gekko japonicus spinal cord.

Bingqiang He, Wenjuan Wang, Chunshuai Sun, Ting Yang, Hui Li, Xiaojun Chen, Hao Liang, Honghua Song, Yongjun Wang, Yingjie Wang

Journal of integrative neuroscience 2021 Sep 30

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Demyelination is one of the pathological outcomes that occur immediately following spinal cord injury. Protection of oligodendrocytes against death/apoptosis proves to be beneficial for the preservation of neurological functions. Suppressors of cytokine signaling 1 protein inhibit the harmful effects of several inflammatory cytokines on oligodendrocytes, but its roles in spinal cord injury (SCI) induced apoptosis of oligodendrocytes remain unclear. We cloned suppressors of cytokine signaling 1 cDNA from Gekko japonicus (Japanese gecko) and analyzed the protein structure revealing the conserved domains contained in other vertebrate suppressors of cytokine signaling 1 proteins. The gecko suppressors of cytokine signaling 1 protein expression were increased in the injured spinal cord following gecko tail amputation and displayed colocalization with oligodendrocytes. The enforced expression of gecko suppressors of cytokine signaling 1 by adenovirus in the Gsn3 gecko oligodendrocyte cell line demonstrated that gecko suppressors of cytokine signaling 1 significantly suppressed cell apoptosis-induced by glucose deprivation. Determination of apoptosis-related proteins revealed that gecko suppressors of cytokine signaling 1 was able to activate extracellular regulated protein kinases (ERK) and serine/threonine protein kinases (Akt). The results presented a distinct protective role of gecko suppressors of cytokine signaling 1 in the regenerative model of the spinal cord, which may provide new cues for central nervous system repair in mammals. © 2021 The Author(s). Published by IMR Press.

Citation

Bingqiang He, Wenjuan Wang, Chunshuai Sun, Ting Yang, Hui Li, Xiaojun Chen, Hao Liang, Honghua Song, Yongjun Wang, Yingjie Wang. Suppressors of cytokine signaling 1 protein in a regenerative model of the Gekko japonicus spinal cord. Journal of integrative neuroscience. 2021 Sep 30;20(3):585-594