USP30-AS1 contributes to mitochondrial quality control in glioblastoma cells.

Ningchao Wang, Jiao Li, Qilei Xin, Naihan Xu

Biochemical and biophysical research communications 2021 Dec 03

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Glioblastoma is the most serious type of brain cancer with poor prognosis. Here, using the publicly available glioma database, we identified that USP30-AS1, an antisense lncRNA locating on the opposite strand of USP30 locus, is upregulated in human gliomas, particularly in high grade glioma. High level of USP30-AS1 is correlated with poor survival in both primary and recurrent glioma patients. USP30-AS1 regulates mitochondrial homeostasis and mitophagy in glioblastoma cells. Knockdown of USP30-AS1 decreases mitochondrial protein expression and mitochondrial mass, promotes mitochondrial uncoupler-induced mitophagy. However, USP30-AS1 does not regulate USP30 expression in a cis-regulatory manner. In summary, this study proposed that USP30-AS1 may serve as a valuable prognostic marker for gliomas. USP3-AS1 is a negative regulator of mitophagy and the regulatory effect is USP30-independent. USP30-AS1 mediated repression of mitophagy may contribute to the loss of mitochondrial homeostasis and tumor development in glioma. Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Citation

Ningchao Wang, Jiao Li, Qilei Xin, Naihan Xu. USP30-AS1 contributes to mitochondrial quality control in glioblastoma cells. Biochemical and biophysical research communications. 2021 Dec 03;581:31-37