Engineering of salt-tolerant Shewanella aquimarina XMS-1 for enhanced pollutants transformation and electricity generation.

Zong-Bin Zhang, Zhou-Hua Cheng, Jing-Hang Wu, Zheng-Bo Yue, Jin Wang, Dong-Feng Liu

The Science of the total environment 2022 Feb 10

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Saline wastewater poses a challenge during bio-treatment process due to salinity affecting the physiological activity of microorganisms and inhibiting their growth and metabolism. Thus, screening and engineering the salt-tolerant strains with stronger performances are urgent. Shewanella aquimarina XMS-1, a salt-tolerant dissimilated metal reducing bacteria (DMRB), was isolated from seawater environment. Its ability for reducing pollutants and generating electricity was enhanced by overexpression of riboflavin synthesis pathway encoding genes from S. oneidensis MR-1 under salt stress. Furthermore, upon contact with graphene oxide (GO), the engineered strain XMS-1/pYYDT-rib with enhanced flavins synthesis could reduce GO and self-assemble to form a three-dimensional (3D) biohybrid system named XMS-1/flavins/rGO. This 3D biohybrid system significantly enhanced the EET efficiency of S. aquimarina XMS-1. Our findings provide a feasible strategy for treatment of salt-containing industrial wastewater contaminated by metal and organic pollutants. Copyright © 2021 Elsevier B.V. All rights reserved.

Citation

Zong-Bin Zhang, Zhou-Hua Cheng, Jing-Hang Wu, Zheng-Bo Yue, Jin Wang, Dong-Feng Liu. Engineering of salt-tolerant Shewanella aquimarina XMS-1 for enhanced pollutants transformation and electricity generation. The Science of the total environment. 2022 Feb 10;807(Pt 3):151009