The expression pattern of CD10 and CD31 identifies fine fibrovascular stroma of grade 1-endometrial endometrioid carcinomas in cytology.

Yoshiaki Norimatsu, Takeshi Nishikawa, Hisae Suzuki, Sho Hosokawa, Hiroko Yano, Yoshinobu Maeda, Tetsuji Kurokawa, Akiko Shinagawa, Tadao K Kobayashi, Franco Fulciniti

Cytopathology : official journal of the British Society for Clinical Cytology 2022 May

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The objective of this study was to assess the diagnostic utility of CD10 in the differential diagnosis of grade 1-endometrial endometrioid carcinoma (G1-EEC) and the metaplastic changes associated with the endometrial glandular and stromal breakdown (EGBD) on liquid-based cytological (LBC) samples. (1) The type and distribution of CD10-positive cells in EGBD and G1-EEC patients were evaluated. (2) Based on the results from (1), histological and cytological specimens were double-immunostained with CD31 and CD10 to confirm whether CD10-positive tubular-canalicular material found in (1) was represented by fine threads of endometrial-type fibrovascular stroma. (3) Based on the results from (2), additional immunostaining of histological specimens was performed for CD146 and αSMA as markers of perivascular cells. (1) CD10 positive cells showed two main patterns of expression: cytoplasmic immunoreactivity in the form of dense brown granules in EGBD and tubular-canalicular branching patterns in G1-EEC. (2) The tubular-canalicular material observed in cytological specimens of G1-EEC samples co-expressed CD10 and CD31, and was interpreted as representing fine threads of endometrial fibrovascular stroma in the corresponding histological samples. Conversely, metaplastic changes in EGBD cases, only a few CD31-positive signals were found inside the condensed stromal clusters with CD10-positive. (3) Cells surrounding the CD31-positive vascular endothelial cells expressed CD146 and αSMA; moreover, some of the thin CD10-positive fibrous stromal strands also co-expressed αSMA. CD10 is a very useful immunomarker for distinguishing between G1-EEC and the metaplastic changes of EGBD in LBC samples. © 2021 John Wiley & Sons Ltd.

Citation

Yoshiaki Norimatsu, Takeshi Nishikawa, Hisae Suzuki, Sho Hosokawa, Hiroko Yano, Yoshinobu Maeda, Tetsuji Kurokawa, Akiko Shinagawa, Tadao K Kobayashi, Franco Fulciniti. The expression pattern of CD10 and CD31 identifies fine fibrovascular stroma of grade 1-endometrial endometrioid carcinomas in cytology. Cytopathology : official journal of the British Society for Clinical Cytology. 2022 May;33(3):362-373