Varsha Singh, Zairah Waris, Ibrahim M Banat, Sriparna Saha, Padmini Padmanabhan
Applied biochemistry and biotechnology 2022 MarA screening programme using mineral salt medium supplemented with n-hexadecane resulted in isolating a Bacillus megaterium SPSW1001 which was capable of producing surface active molecules lowering culture medium surface tension to 27.43 ± 0.029 mN/m and interfacial tension to 0.38 ± 0.03 mN/m at 72 h and an emulsification index (E24) (85.63%). The biosurfactant product was further used to assess its effects on the rheological characteristics of water-in-oil emulsion prepared with engine oil. Structural characterization of the biosurfactant product by FTIR revealed a C-O-C stretch in sugar moiety and ester carbonyl linkage group between sugar and fatty acids, respectively, while mass spectral analysis revealed its glycolipid nature, with an m/z value of 662.44. The fluid behaviour of water-in-oil emulsion showed a non-Newtonian viscoelastic dilatant flow after yielding exemplified appropriately by Herschel-Bulkley model with 100% confidence of fit. The present study is significant in formulation and handling, processing, and transport of emulsion and in understanding flocculation characteristics. © 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Varsha Singh, Zairah Waris, Ibrahim M Banat, Sriparna Saha, Padmini Padmanabhan. Assessment of Rheological Behaviour of Water-in-Oil Emulsions Mediated by Glycolipid Biosurfactant Produced by Bacillus megaterium SPSW1001. Applied biochemistry and biotechnology. 2022 Mar;194(3):1310-1326
PMID: 34694553
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