Development of a Simple Dansyl-Based PH Fluorescent Probe in Acidic Medium and Its Application in Cell Imaging.

Yu Liu, Yan Wang, Li Zhao, Baocai Xu

Journal of fluorescence 2022 Jan

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A simple pH fluorescent probe based on dansyl derivative (Bu-Dns) was synthesized via one-step reaction between dansyl chloride and 4-bromobutan-1-amine hydrobromide. The obtained probe showed good selectivity and sensitivity toward H+ in acidic medium over two pH units (~4-2). At pH > 4, Bu-Dns solution emitted yellow fluorescent light, which became gradually weaker with decreasing pH value. At pH below 2, complete fluorescence quenching occurred. The pH response of Bu-Dns was ascribed to the protonation of dimethylamine group. The lack of influence of metal ion on pH response increases the prevalence of Bu-Dns in the potential detection of pH variation in acidic aqueous media. More importantly, it can sense the intracellular pH change in acidic range. © 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Yu Liu, Yan Wang, Li Zhao, Baocai Xu. Development of a Simple Dansyl-Based PH Fluorescent Probe in Acidic Medium and Its Application in Cell Imaging. Journal of fluorescence. 2022 Jan;32(1):227-233