Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon Sequencing.

Alejandro R Walker, Robert C Shields

Methods in molecular biology (Clifton, N.J.) 2022

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Transposon sequencing (Tn-seq) has greatly accelerated the rate at which gene function can be profiled in microbial organisms. This technique has been applied to the study of the dental caries pathogen Streptococcus mutans where it has been used to generate large transposon mutant libraries. Coupled with high-throughput sequencing and bioinformatics tools, culture of these transposon mutant libraries has facilitated the identification of essential and conditional essential genes. In this chapter, we describe a procedure for performing Tn-seq studies in S. mutans that covers pooled transposon mutant construction, in vitro culture, and DNA library sequencing and data analysis. © 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Alejandro R Walker, Robert C Shields. Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon Sequencing. Methods in molecular biology (Clifton, N.J.). 2022;2377:237-258