Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin against Daptomycin-Nonsusceptible Enterococcus faecium.
Taylor Morrisette, Katherine L Lev, Gregory S Canfield, Breck A Duerkop, Razieh Kebriaei, Kyle C Stamper, Dana Holger, Susan M Lehman, Smaranda Willcox, Cesar A Arias, Michael J Rybak
Antimicrobial agents and chemotherapy 2022 Jan 18Enterococcus faecium is a significant multidrug-resistant pathogen. Bacteriophage cocktails are being proposed to complement antibiotic therapy. After a screen of 8 E. faecium strains against 4 phages, 2 phages (113 and 9184) with the broadest host ranges were chosen for further experiments. Transmission electron microscopy, whole-genome sequencing, comparative genome analyses, and time-kill analyses were performed. Daptomycin (DAP) plus the phage cocktail (113 [myophage] and 9184 [siphopage]) showed bactericidal activity in most regimens, while DAP addition prevented phage 9184 resistance against daptomycin-nonsusceptible E. faecium.
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Taylor Morrisette, Katherine L Lev, Gregory S Canfield, Breck A Duerkop, Razieh Kebriaei, Kyle C Stamper, Dana Holger, Susan M Lehman, Smaranda Willcox, Cesar A Arias, Michael J Rybak. Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin against Daptomycin-Nonsusceptible Enterococcus faecium. Antimicrobial agents and chemotherapy. 2022 Jan 18;66(1):e0162321
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PMID: 34723631
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