A simple and fast LC-MS/MS method for the simultaneous determination of tenofovir alafenamide and tenofovir in human plasma.

Xiaoping Qian, Qi Chen, Yunhui Chen, Shiliang Ji, Ying Wang, Ye Sun, Huixin Qi, Kan Zhong, Jinfang Jiang, Xin Chen, Lifeng Huang, Yong Yang

Biomedical chromatography : BMC 2022 Mar

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A simple and fast liquid chromatography-tandem mass spectrometry method was established and validated for the simultaneous determination of tenofovir alafenamide (TAF) and tenofovir (TNF) in human plasma. A simple protein precipitation procedure was employed to extract analytes from plasma. Chromatographic separation was performed on an Eclipse Plus C18 column utilizing a fast gradient elution starting with 2% of 2 mM ammonium acetate-formic acid (100/0.1, v/v) followed by increasing the percentage of acetonitrile. Detection was performed on a tandem mass spectrometer equipped with an electrospray ionization source operated in the positive ionization mode, using the transitions m/z 477.2 → m/z 346.1 for TAF and m/z 288.1 → m/z 176.1 for TNF. TAF-d5 and TNF-d7 were used as the internal standard of TAF and TNF, respectively. The method was validated in the concentration ranges 1.25-500 ng/mlfor TAF and 0.300-15.0 ng/ml for TNF with acceptable accuracy and precision. © 2021 John Wiley & Sons, Ltd.

Citation

Xiaoping Qian, Qi Chen, Yunhui Chen, Shiliang Ji, Ying Wang, Ye Sun, Huixin Qi, Kan Zhong, Jinfang Jiang, Xin Chen, Lifeng Huang, Yong Yang. A simple and fast LC-MS/MS method for the simultaneous determination of tenofovir alafenamide and tenofovir in human plasma. Biomedical chromatography : BMC. 2022 Mar;36(3):e5273