miR-34a-5p blocks cervical cancer growth and migration by downregulating CDC25A.

To define mechanisms underlying the regulation of CDC25A by miR-34a-5p in cervical cancer (CC). Quantifications of miR-34a-5p and CDC25A levels were performed in 68 CC tissues and matched controls. CC cell lines HeLa and SiHa were transfected to monitor their biological behavior changes. Low-expressed miR-34a-5p and up-regulated CDC25A were noted in CC. Either overexpression of miR-34a-5p or inhibition of CDC25A blocked the growth and migration of HeLa and SiHa cells, resulting in an increase in apoptosis (p<0.05), while inhibition of miR-34a-5p or overexpression of CDC25A promoted tumor growth. Dual-luciferase reporter (DLR) assay and cell transfection identified the targeting relationship between miR-34a-5p and CDC25A, and correlation analysis revealed a negative association between them in CC. Co-transfection assays showed that overexpression of CDC25A reversed the inhibition of miR-34a-5p on CC growth and migration. miR-34a-5p mediates growth, migration and other malignant behaviors in CC by regulating CDC25A.

Citation

Tao Jiang, Hongyan Cheng. miR-34a-5p blocks cervical cancer growth and migration by downregulating CDC25A. Journal of B.U.ON. : official journal of the Balkan Union of Oncology. 2021 Sep-Oct;26(5):1768-1774

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PMID: 34761581

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