Toxocara DNA amplification in serum and tissue samples in BALB/c mice.

Gabriela Rodrigues E Fonseca, Gessica Baptista de Melo, Fabiana Martins de Paula, Fernanda Mello Malta, Ronaldo Cesar Borges Gryschek, Susana Angélica Zevallos Lescano

Molecular and biochemical parasitology 2021 Nov

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Toxocariasis is still a neglected parasitic disease worldwide and much about its biology and diagnosis has yet to be understood. The migration of third stage larvae via bloodstream suggests a potential use of molecular tools in diagnosis as well to deepen the knowledge about its migration behaviors. Conventional PCR was applied in serum and tissue samples from BALB/c mice infected with 5 and 500 embryonated eggs. Blood samples were collected at 15, 30, 60, 90 and 120 days post-infection. Organs were excised at 170 days post infection. There was no DNA amplification in serum samples in any group or day post-infection; contrarily, tissue samples showed DNA amplification. These results also support a continuous larval migration after and/or simultaneously with the neurotropic-myotropic phase. Thus, molecular tools might be useful as a differential diagnosis method, but do not replace immunodiagnostics techniques. Copyright © 2021 Elsevier B.V. All rights reserved.

Citation

Gabriela Rodrigues E Fonseca, Gessica Baptista de Melo, Fabiana Martins de Paula, Fernanda Mello Malta, Ronaldo Cesar Borges Gryschek, Susana Angélica Zevallos Lescano. Toxocara DNA amplification in serum and tissue samples in BALB/c mice. Molecular and biochemical parasitology. 2021 Nov;246:111429