Correlation Engine 2.0
Clear Search sequence regions

  • cell density (1)
  • drosophila (2)
  • furin (2)
  • GPIHBP1 (4)
  • human (5)
  • lipid (1)
  • lipoprotein (2)
  • LPL (16)
  • mice (1)
  • Sizes of these terms reflect their relevance to your search.

    LPL is essential for intravascular lipid metabolism and is of high medical relevance. Since LPL is notoriously unstable, there is an unmet need for a robust expression system producing high quantities of active and pure recombinant human LPL (hLPL). We showed previously that bovine LPL purified from milk is unstable at body temperature (Tm is 34.8°C), but in the presence of the endothelial transporter glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 (GPIHBP1), LPL is stabile (Tm increases to 57.6°C). Building on this information, we now designed an expression system for hLPL using Drosophila Schneider 2 cells grown in suspension at high cell density and at an advantageous temperature of 25°C. We cotransfected Schneider 2 cells with hLPL, lipase maturation factor 1, and soluble GPIHBP1 to provide an efficient chaperoning and stabilization of LPL in all compartments during synthesis and after secretion into the conditioned medium. For LPL purification, we used heparin-Sepharose affinity chromatography, which disrupted LPL-GPIHBP1 complexes causing GPIHBP1 to elute with the flow-through of the conditioned media. This one-step purification procedure yielded high quantities of pure and active LPL (4-28 mg/l). Purification of several hLPL variants (furin cleavage-resistant mutant R297A, active-site mutant S132A, and lipid-binding-deficient mutant W390A-W393A-W394A) as well as murine LPL underscores the versatility and robustness of this protocol. Notably, we were able to produce and purify LPL containing the cognate furin cleavage site. This method provides an efficient and cost-effective approach to produce large quantities of LPL for biophysical and large-scale drug discovery studies. Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.


    Anne-Marie Lund Winther, Kristian Kølby Kristensen, Anni Kumari, Michael Ploug. Expression and one-step purification of active LPL contemplated by biophysical considerations. Journal of lipid research. 2021;62:100149

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 34780727

    View Full Text