Xue-Qi Liu, Xin-Rong Shao, Ye Liu, Zhao-Xia Dong, Sze-Hoi Chan, Yuan-Yuan Shi, Shu-Na Chen, Lin Qi, Li Zhong, Yue Yu, Ting Lv, Peng-Fei Yang, Li-Yan Li, Xiao-Bin Wang, Xu-Dong Zhang, Xin Li, Wenxue Zhao, Lalit Sehgal, Miao Li, Xing-Ding Zhang
Oncogene 2022 JanBladder cancer (BLCA) is the most common malignant tumor of the urinary system and is characterized by high metastatic rates and poor prognosis. The expression of tight junction protein 1 (TJP1) is associated with bladder cancer invasion; however, the mechanism by which TJP1 affects vasculature remodeling remains unknown. In this study, we found that TJP1 expression correlated with tumor angiogenesis and poor overall survival in clinical samples. Furthermore, TJP1 overexpression promoted tumor angiogenesis in BLCA cells and stimulated recruitment of macrophages to tumors by upregulating CCL2 expression. Mechanistically, TJP1 interacted with TWIST1 and enhanced the transcriptional activity of CCL2. The impairment of tumor angiogenesis caused by knockdown of TJP1 was dramatically rescued by overexpression of TWIST1. Furthermore, TJP1 recruited USP2, which deubiquitinated TWIST1, thereby protecting TWIST1 from proteasome-mediated protein degradation. In conclusion, our results suggest that TJP1 controls angiogenesis in BLCA via TWIST1-dependent regulation of CCL2. We demonstrate that TJP1 functions as a scaffold for the interaction between USP2 and TWIST1 and this may provide potential therapeutic targets in bladder cancer. © 2021. The Author(s), under exclusive licence to Springer Nature Limited.
Xue-Qi Liu, Xin-Rong Shao, Ye Liu, Zhao-Xia Dong, Sze-Hoi Chan, Yuan-Yuan Shi, Shu-Na Chen, Lin Qi, Li Zhong, Yue Yu, Ting Lv, Peng-Fei Yang, Li-Yan Li, Xiao-Bin Wang, Xu-Dong Zhang, Xin Li, Wenxue Zhao, Lalit Sehgal, Miao Li, Xing-Ding Zhang. Tight junction protein 1 promotes vasculature remodeling via regulating USP2/TWIST1 in bladder cancer. Oncogene. 2022 Jan;41(4):502-514
PMID: 34782718
View Full Text