Biofunctionalisation of gallium arsenide with neutravidin.

Gallium arsenide (GaAs) is a promising candidate as a platform for optical biosensing devices due to its enabling optoelectronic properties. However, the biofunctionalisation of the GaAs surface has not received much attention compared to gold, carbon and silicon surfaces. Here we report a study presenting a physicochemical surface characterisation of the GaAs surface along the functionalisation with a high-affinity bioconjugation pair widely explored in the life sciences - biotin and neutravidin. Combined X-ray photoelectron spectroscopy (XPS), wettability measurements and spectroscopic ellipsometry were used for a reliable characterisation of the surface functionalisation process. The results suggest that a film with a thickness lower than 10 nm was formed, with a neutravidin to biotin ratio of 1:25 on the GaAs surface. Reduction of non-specific binding of the protein to the surface was achieved by optimising the protein buffer and rinsing steps. This study shows the feasibility of using GaAs as a platform for specific biomolecular recognition, paving the way to a new generation of optoelectronic biosensors. Copyright © 2021 Elsevier Inc. All rights reserved.

Citation

Bárbara Santos Gomes, David J Morgan, Wolfgang Langbein, Paola Borri, Francesco Masia. Biofunctionalisation of gallium arsenide with neutravidin. Journal of colloid and interface science. 2022 Feb 15;608(Pt 3):2399-2406

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PMID: 34794804

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