CD62L expression level determines the cell fate of myeloid progenitors.

Yusuke Ito, Fumio Nakahara, Yuki Kagoya, Mineo Kurokawa

Stem cell reports 2021 Dec 14

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Hematopoietic cells differentiate through several progenitors in a hierarchical manner, and recent single-cell analyses have revealed substantial heterogeneity within each progenitor. Although common myeloid progenitors (CMPs) are defined as a multipotent cell population that can differentiate into granulocyte-monocyte progenitors (GMPs) and megakaryocyte-erythrocyte progenitors (MEPs), and GMPs generate neutrophils and monocytes, these myeloid progenitors must contain some lineage-committed progenitors. Through gene expression analysis at single-cell levels, we identified CD62L as a marker to reveal the heterogeneity. We confirmed that CD62L-negative CMPs represent "bona fide" CMPs, whereas CD62L-high CMPs are mostly restricted to GMP potentials both in mice and humans. In addition, we identified CD62L-negative GMPs as the most immature subsets in GMPs and Ly6C+CD62L-intermediate and Ly6C+CD62L-high GMPs are skewed to neutrophil and monocyte differentiation in mice, respectively. Our findings contribute to more profound understanding about the mechanism of myeloid differentiation. Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Citation

Yusuke Ito, Fumio Nakahara, Yuki Kagoya, Mineo Kurokawa. CD62L expression level determines the cell fate of myeloid progenitors. Stem cell reports. 2021 Dec 14;16(12):2871-2886