CRISPR/Cas9 editing in conditionally immortalized HoxB8 cells for studying gene regulation in mouse dendritic cells.

Huaming Xu, Thomas Look, Sujeethkumar Prithiviraj, Daniel Lennartz, Manuel Delgado Cáceres, Katrin Götz, Paul Wanek, Hans Häcker, Rafael Kramann, Kristin Seré, Martin Zenke

European journal of immunology 2022 Nov

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HoxB8 multipotent progenitors (MPP) are obtained by expression of the estrogen receptor hormone binding domain (ERHBD) HoxB8 fusion gene in mouse BM cells. HoxB8 MPP generate (i) the full complement of DC subsets (cDC1, cDC2, and pDC) in vitro and in vivo and (ii) allow CRISPR/Cas9-mediated gene editing, for example, generating homozygous deletions in cis-acting DNA elements at high precision, and (iii) efficient gene repression by dCas9-KRAB for studying gene regulation in DC differentiation. © 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.

Citation

Huaming Xu, Thomas Look, Sujeethkumar Prithiviraj, Daniel Lennartz, Manuel Delgado Cáceres, Katrin Götz, Paul Wanek, Hans Häcker, Rafael Kramann, Kristin Seré, Martin Zenke. CRISPR/Cas9 editing in conditionally immortalized HoxB8 cells for studying gene regulation in mouse dendritic cells. European journal of immunology. 2022 Nov;52(11):1859-1862