miR-1290 promotes IL-8-mediated vascular endothelial cell adhesion by targeting GSK-3β.

MicroRNA-1290 (miR-1290) has been reported to be involved in many diseases and play a key role during the development process. However, the role of miR-1290 in atherosclerosis (AS) is still unclear. The current study showed that the expressions of miR-1290 were high in serum of patients with hyperlipidemia. The functional role of miR-1290 were then investigated in human umbilical vein endothelial cells (HUVECs). Here, we found that miR-1290 expressions were notably enhanced in HUVECs mediated by IL-8. miR-1290 inhibitor repressed monocytic THP-1 cells adhesion to HUVECs by regulating ICAM-1 and VCAM-1, inhibited proliferation through regulating cyclinD1 and PCNA, and inhibited inflammatory response by regulating IL-1β. Mechanistically, we verified that miR-1290 mimic was able to directly target the 3'-UTR of GSK-3β mRNA using luciferase reporter assay. Knockdown of GSK-3β (si-GSK-3β) promoted HUVECs adhesion and the expression of IL-1β, and partially restore the depression effect of miR-1290 inhibitor on HUVECs adhesion and inflammation. In contrast, si-GSK-3β inhibited the proliferation of HUVECs and the expression of cyclinD1 and PCNA. In summary, our study revealed that miR-1290 promotes IL-8-mediated the adhesion of HUVECs by targeting GSK-3β. However, GSK-3β is not the target protein for miR-1290 to regulate the proliferation of HUVECs. Our findings may provide potential target in atherosclerosis treatment. © 2021. The Author(s), under exclusive licence to Springer Nature B.V.

Citation

Hongxin Xu, Ying Cui, Xianwei Liu, Xiao Zheng, Jiaqing Liu, Xinxin Hu, Fuhua Gao, Xiaoyan Hu, Mei Li, Xiaoqing Wei, Ying Gao, Ying Zhao. miR-1290 promotes IL-8-mediated vascular endothelial cell adhesion by targeting GSK-3β. Molecular biology reports. 2022 Mar;49(3):1871-1882

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PMID: 34837150

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