Liposomes encapsulating artificial cytosol as drug delivery system.

Wei Zong, Xiaotong Shao, Yunhe Chai, Xiuwen Wang, Shuang Han, Hongtao Chu, Chuntao Zhu, Xunan Zhang

Biophysical chemistry 2022 Feb

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The fabrication of cell models containing artificial cytosol is challenging. Herein we constructed an artificial cytosol contained cell model by electroformation method. Agarose was selected as the main component of the artificial cytosol, and sucrose was added into the agarose to regulate the sol viscosity and the phase transition temperature. The viscosity of the sol with the mass ratio (agarose-sucrose) 1:9 was closest to the natural cytosol. DSPC/20 mol% cholesterol was used to form large unilamellar vesicles (LUVs) as cell model compartment. The rhodamine release experiment confirmed that the unique release profile of agarose-sucrose@LUVs is suitable as a drug carrier. Doxorubicin is loaded in the agarose-sucrose@LUVs, and their half maximum inhibition concentration on HeLa cells is 0.016 μmol L-1, which means 28.7 times increase in inhibition efficiency over free doxorubicin. Copyright © 2021 Elsevier B.V. All rights reserved.

Citation

Wei Zong, Xiaotong Shao, Yunhe Chai, Xiuwen Wang, Shuang Han, Hongtao Chu, Chuntao Zhu, Xunan Zhang. Liposomes encapsulating artificial cytosol as drug delivery system. Biophysical chemistry. 2022 Feb;281:106728