Ruyi He, Longyu Wang, Fei Wang, Jun Yang, Xiao Yu, Yuan Wang, Zhiguo Liu, Chunhua Li, Lixin Ma
The Analyst 2021 Dec 20A simple and user-friendly nucleic acid sensing platform with 10 aM sensitivity, named USPCRP (combines ultrashort PCR with Pyrococcus furiosus Argonaute cleavage for nuleic acids detection) is reported. The product of this ultrashort PCR could be directly used as a DNA guide to mediate PfAgo cleavage of molecular beacons.
Ruyi He, Longyu Wang, Fei Wang, Jun Yang, Xiao Yu, Yuan Wang, Zhiguo Liu, Chunhua Li, Lixin Ma. Combination of ultrashort PCR and Pyrococcus furiosus Argonaute for DNA detection. The Analyst. 2021 Dec 20;147(1):35-39
PMID: 34881761
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