A flow-through chromatography purification process for Vero cell-derived influenza virus (H7N9).

ChengRui Fei, JingXia Gao, ChengHua Fei, Lei Ma, WenYong Zhu, LingYu He, YaNan Wu, ShaoHui Song, WeiDong Li, Jian Zhou, GuoYang Liao

Journal of virological methods 2022 Mar

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Immunization is the most effective way to respond to an influenza epidemic. To produce Vero cell-derived influenza vaccines, a more efficient, stable and economical purification process is required. In this study, we purified the H7N9 influenza virus grown in Vero cells that were cultured in a serum-free medium by using a combination of anion exchange chromatography (AEC) and ligand-activated core chromatography (LCC), which avoids the virus capture step. After purification, 99.95 % host cell DNA (hcDNA) (final concentration: 28.69 pg/dose) and 98.87 % host cell protein (HCP) (final concentration: 28.28 ng/dose) were removed. The albumin content was 11.36 ng/dose. All these remnants met the current Chinese Pharmacopoeia and WHO requirements. The final virus recovery rate was 58.74 %, with the concentration of hemagglutinin recorded at 132.12 μg/mL. The flow-through chromatography purification process represents an alternative to the existing processes for cell-derived influenza viruses and might be suitable for the purification of other viruses as well. Copyright © 2021 Elsevier B.V. All rights reserved.

Citation

ChengRui Fei, JingXia Gao, ChengHua Fei, Lei Ma, WenYong Zhu, LingYu He, YaNan Wu, ShaoHui Song, WeiDong Li, Jian Zhou, GuoYang Liao. A flow-through chromatography purification process for Vero cell-derived influenza virus (H7N9). Journal of virological methods. 2022 Mar;301:114408