Long-Term Preservation of Plant Viruses in Cryopreserved Shoot Tips.

Availability of the methods for long-term virus preservation facilitates easy acquirement of viruses, which are needed in many basic and applied virological studies. Cryopreservation is currently considered an ideal means for long-term preservation of plant germplasm. Recent studies have shown that cryopreservation provided an efficient and reliable method for long-term preservation of plant viruses. Here, we describe the detailed procedures of droplet vitrification for long-term preservation of apple stem grooving virus (ASGV), which represents a type of viruses that can invade meristematic cells of the shoot tips, and potato leafroll virus (PLRV), which is a phloem-limited virus that does not infect the apical meristem. Shoot tip cryopreservation provides an advantageous strategy for the long-term preservation of plant viruses. © 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Min-Rui Wang, Tianxing Pang, Ziqian Lian, Qiao-Chun Wang, Liying Sun. Long-Term Preservation of Plant Viruses in Cryopreserved Shoot Tips. Methods in molecular biology (Clifton, N.J.). 2022;2400:187-195

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PMID: 34905202

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