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    The polymerase chain reaction (PCR) can be used to produce both nonradiolabeled DNA probes and radiolabeled DNA probes with high specific activity. In this protocol, PCR is used to generate double-stranded probes. Related methods, including the generation of asymmetric probes by PCR, are also discussed. © 2022 Cold Spring Harbor Laboratory Press.


    Michael R Green, Joseph Sambrook. Labeling of DNA Probes by Polymerase Chain Reaction. Cold Spring Harbor protocols. 2022 Mar 01;2022(3)

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    PMID: 34907078

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