A one-step reverse transcription recombinase polymerase amplification assay for lateral flow-based visual detection of PVY.

Arabelle Cassedy, Michele Della Bartola, Anne Parle-McDermott, Ewen Mullins, Richard O'Kennedy

Analytical biochemistry 2022 Apr 01

filter terms:

Potato virus Y (PVY) is an abundant and damaging virus which reduces crop yield and marketability. Accurate detection of this economically important virus both in-field and in seed potatoes is considered essential in the control of PVY spread. Current detection methods are focused on immunodetection and PCR-based methods, however, identification of PVY through isothermal amplification is a promising avenue for developing accessible, on-site diagnostics with quick turnaround times. In this work, a rapid recombinase polymerase amplification assay was developed which could readily amplify PVY nucleic acids with good sensitivity and specificity. Additionally, this assay was shown to be capable of amplification directly from RNA in a one-step amplification process, without the need for prior reverse transcription. The assay was coupled with lateral flow technology to provide a rapid visual confirmation of amplification. This nucleic-acid lateral flow immunoassay could feasibly be employed in-field, or at any location where testing is required, to aid in the detection and control of PVY. Copyright © 2021 Elsevier Inc. All rights reserved.

Citation

Arabelle Cassedy, Michele Della Bartola, Anne Parle-McDermott, Ewen Mullins, Richard O'Kennedy. A one-step reverse transcription recombinase polymerase amplification assay for lateral flow-based visual detection of PVY. Analytical biochemistry. 2022 Apr 01;642:114526