Validation of the usefulness of 26S rDNA D1/D2, internal transcribed spacer, and intergenic spacer 1 for molecular epidemiological analysis of Macrorhabdus ornithogaster.

Atsushi Kojima, Nanako Osawa, Mami Oba, Yukie Katayama, Tsutomu Omatsu, Tetsuya Mizutani

The Journal of veterinary medical science 2022 Feb 23

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Macrorhabdus ornithogaster (MO) is an infectious fungus that causes gastric damage in birds. In this study, we established nested and seminested polymerase chain reaction (PCR) methods that specifically amplify the domain D1/D2 region (D1/D2) of 26S ribosomal DNA (rDNA), internal transcribed spacer (ITS) of rDNA, and intergenic spacer (IGS) 1 region from avian feces. Phylogenetic analysis of MO collected from Japanese pet birds showed little genetic variation; analysis based on these regions did not distinguish between host species order, differences in MO shape, or host gastrointestinal symptoms. These regions were found to be unsuitable for molecular epidemiological studies of MO and further investigation into other genetic regions is required.

Citation

Atsushi Kojima, Nanako Osawa, Mami Oba, Yukie Katayama, Tsutomu Omatsu, Tetsuya Mizutani. Validation of the usefulness of 26S rDNA D1/D2, internal transcribed spacer, and intergenic spacer 1 for molecular epidemiological analysis of Macrorhabdus ornithogaster. The Journal of veterinary medical science. 2022 Feb 23;84(2):244-250