Chromatin accessibility and microRNA expression in nephron progenitor cells during kidney development.

Andrew Clugston, Andrew Bodnar, Débora Malta Cerqueira, Yu Leng Phua, Alyssa Lawler, Kristy Boggs, Andreas R Pfenning, Jacqueline Ho, Dennis Kostka

Genomics 2022 Jan

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Mammalian nephrons originate from a population of nephron progenitor cells, and changes in these cells' transcriptomes contribute to the cessation of nephrogenesis, an important determinant of nephron number. To characterize microRNA (miRNA) expression and identify putative cis-regulatory regions, we collected nephron progenitor cells from mouse kidneys at embryonic day 14.5 and postnatal day zero and assayed small RNA expression and transposase-accessible chromatin. We detect expression of 1104 miRNA (114 with expression changes), and 46,374 chromatin accessible regions (2103 with changes in accessibility). Genome-wide, our data highlight processes like cellular differentiation, cell migration, extracellular matrix interactions, and developmental signaling pathways. Furthermore, they identify new candidate cis-regulatory elements for Eya1 and Pax8, both genes with a role in nephron progenitor cell differentiation. Finally, we associate expression-changing miRNAs, including let-7-5p, miR-125b-5p, miR-181a-2-3p, and miR-9-3p, with candidate cis-regulatory elements and target genes. These analyses highlight new putative cis-regulatory loci for miRNA in nephron progenitors. Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Citation

Andrew Clugston, Andrew Bodnar, Débora Malta Cerqueira, Yu Leng Phua, Alyssa Lawler, Kristy Boggs, Andreas R Pfenning, Jacqueline Ho, Dennis Kostka. Chromatin accessibility and microRNA expression in nephron progenitor cells during kidney development. Genomics. 2022 Jan;114(1):278-291