The Piragua Gene Is not Essential for Drosophila Development.

Proteins with clusters of C2H2 zinc finger domains (C2H2-proteins) constitute the most abundant class of transcription factors in higher eukaryotes. N-terminal ZAD (zinc finger-associated domain) dimerization domain has been identified in a large group of C2H2-proteins mostly in insects. The piragua gene encodes one of these proteins, Fu2. We have generated CRISPR/Cas9-mediated deletion of the piragua gene that has no phenotype. We have used φC31-mediated attP/attB recombination to generate a transgenic line expressing Fu2 protein fused with HA epitope. This line will be useful for analysis of DNA binding profile and functions of Fu2 protein. © 2021. Pleiades Publishing, Ltd.

Citation

V V Sokolov, P G Georgiev, O V Kyrchanova. The Piragua Gene Is not Essential for Drosophila Development. Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections. 2021 Nov;501(1):197-200

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PMID: 34962606

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